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FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN LOQUAT

2011

The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars. Protocols were developed for isolation and culture of loquat microspores. Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures. After 4 weeks of culture, the responding microspores produced yellowish/white callus. The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.

MaloideaeRosaceaefungifood and beveragesEriobotrya japonica cell structures haploid pollen embryogenesisEriobotryaHorticultureBiologybiology.organism_classificationJaponicaPollen embryogenesisEriobotrya japonicaMicrosporeCallusBotanyDoubled haploidyCell structureHaploidPloidyActa Horticulturae

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